Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Quant-iT RNA BR (Broad Range) Assay Kit. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Regulations & Guidance; Manuals 0 0. Double stranded DNA quantification performed in down to 2 µl using the LVis Plate. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). P7589. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. High tolerance for contaminants. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. ただ、この製品のマニュアルには蛍光分光光度計での測定方法しか. Similar. Quantitation Range. BR reagent (Component A) 1. BR reagent (Component A) 1. Rapidly detect as little as 0. This was possible since, due to the low height of the light ray of only 8. Rapidly detect as little as 0. The Molecular Probes PicoGreen®. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 50 pg to 2 µg. High linearity and sensitivity over a broad DNA range. Picogreen assays are compatible with most microplate readers and fluorometers. Skip to main list 美谷分子仪器（上海）有限公司. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. Tweet. Rapidly detect as little as 0. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. Table 2 summarizes results of the. Quant-iT PicoGreen dsDNA Assay Kit. After a 5-minute. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Add 5uL of PicoGreen reagent for every 1mL of buffer. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Reagent. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. Pico488 allows to quantitate as little as low pg/mL of dsDNA. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Table 2 summarizes results of the. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. 文件. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. For Research Use. Catalog Number. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. This protocol is a simplified and condensed. Product Type. Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. No. PicoGreen®試薬アッセイ. 2–100 ng: 2-1,000 ng: 0. 2. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Determination of DNA Yield. DNA standard curve. Q33130). Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. Picogreen assays are compatible with most microplate readers and fluorometers. 2-200 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iTTM DNA Assay Kit, Broad Range 2-1000 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iT™ DNA Assay Kit, High Sensitivity 0. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. Mix PicoGreen and DNA by pippetting up and down 5-10 times. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT RiboGreen RNA Reagent The Quant-iT RiboGreen RNA Reagent is one of the most sensitive fluorescent dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. Quant-iT™ PicoGreen ® dsDNA Assay Kit. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). • Suitable for use in 96- and 384-well plate formats. Reagent Search & Data Services Menu Reagent Search. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 0. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Unit Size. This protocol is a simplified and condensed. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. 1. Table 2 summarizes results of the. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Rapidly detect as little as 0. Quant-iT Assay Kits Quant-iT DNA Assay Kit, High Sensitivity The Quant-iT High-Sensitivity DNA Assay Kit has a linear detection range of 0. Buy from Supplier. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). 10 ng/mkl – 10000 ng/ml. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Control samples were not lysed and consisted of free pDNA. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. Bioz Stars score: 86/100, based on 1 PubMed citations. Rapidly detect as little as 0. Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. For a standard. 8. Highly selective for dsDNA over ssDNA or RNA. Results: DNA concentration values were measured by the “DNA Mamm. '. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Fluorescence was measured at. Rapidly detect as little as 0. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. Fluorometric dsDNA Assay. PicoGreen dsDNA quantitation reagents are. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. This protocol is a simplified and condensed version of. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. The Rapidly detect as little as 0. 10 x 100 µL. • Suitable for use in 96- and 384-well plate formats. assay. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). Touch devices users use touch & swipe gestures. Thermo Fisher quant it picogreen dsdna quantification reagent kit Quant It Picogreen Dsdna Quantification Reagent Kit, supplied by Thermo Fisher, used in various techniques. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. Related applications: Nucleic Acid Quantitation. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. DNA was quantified by the Quant-it PicoGreen dsDNA Assay Kit (Invitrogen Life technologies, UK) according to the manufacturer’s protocol. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. Materials: 1. You will need 100 μl diluted PicoGreen reagent per well. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. Quant It Picogreen Dsdna Quantitation Assay Kit, supplied by Thermo Fisher, used in various techniques. Picogreen assay kit (Invitrogen, P7589) a. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Rapidly detect as little as 0. Free dye. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. お問い合わせ一覧 . Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). PicoGreen dsDNA quantitation reagents are. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. • Specific for dsDNA in the presence of RNA. Quantification and normalization of double stranded DNA with minimal set-up time. 50 pg to 2 µg. Price from $9. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. Got it. Abstract. PicoGreen dsDNA quantitation reagents are. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. Quant-iT PicoGreen dsDNA Assay Kit. The Qubit dsDNA BR Assay kit contains a highly selective enhancing reagent specific for quantifying dsDNA using volumes as low as 1 µL in a standard 96-well microplate format. Rapidly detect as little as 0. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. Barcoded amplicons were pooled in. Highly selective for dsDNA over ssDNA or RNA. The use of a serial dilution of dsDNA standards whileThe quantity of ChIP DNA should first be determined with the Quant-iT™ PicoGreen dsDNA Reagent Kit (Invitrogen). • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Picogreen assays are compatible with most microplate readers and fluorometers. Common contaminants such as salts, free nucleotides, solvents,Product Description. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 1 mL kit. No. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 125 to 100 ng/mL. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 0 mL to 200 uL to fit a 96-well microplate format. PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Unit Size. Catalog Number. Thermo Fisher quant it picogreen dsdna quantitation assay kit. The tubes were. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. 99. For duplicate-well droplet. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. ITSkabir). VIEWS. g. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 1. 15日开始对本试剂盒内标品（B组分）进行更新，由原来的小牛胸腺DNA升级为Lamda DNA，内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. 5). • Suitable for use in 96- and 384-well plate formats. P11496). Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. 1X dsDNA assay (%) 100 17. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). 2 – 100 ng. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 10 x 100 µL. • Specific for dsDNA in the presence of RNA. 7 Q33231 6 1,000 41. Each sample was amplified in triplicate, and the triplicate amplicons were pooled and purified by gel extraction and quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, P7589). In case dsDNA standards are available only the quantification reagent can be used. 22. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. with the Qubit dsDNA BR Assay Kit and 1 µL of each sample was measured in quadruplicate with the Quant-iT™ PicoGreen dsDNA Assay Kit. Quant-iTTM PicoGreen® Assay Reagent 0. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. yields in a method for direct cycle sequencing of PCR products. 2. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. The following protocol is an adaptation of the Mo-lecular ProbesTM dsDNA Quantitation Reagent and Kits product information sheet. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. Q33120) • Quant-iT dsDNA Assay Kit, broad range (Cat. 作成者 LatB Staff 01. The normalized DNA was aliquoted into multiple tubes at 50µL volume. 12. The Quant-iT PicoGreen dsDNA. Qubit DNA and RNA assays are available with different quantification ranges. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Picogreen assays are compatible with most microplate readers and fluorometers. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. Prepare the assay. Picogreen assays are compatible with most microplate readers and fluorometers. Price from $9. Per Well Volume 150 uL/well. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Picogreen 定量试剂盒兼容大多数微孔板读数仪和荧光计。. • Specific for dsDNA in the presence of RNA. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. P11495. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. 参考資料. Quant-iT PicoGreen dsDNA Assay Kit a. Catalog number: P7581. PicoGreen dsDNA quantitation reagents are. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. 50 pg to 2 µg. Picogreen assays are compatible with most microplate readers and fluorometers. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 2. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Product Type. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. (2012). • Suitable for use in 96- and 384-well plate formats. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. Quantify nucleic acids and proteins using four user-selectable fluorescent. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. UV흡광 결과보다 민감도 우수. Quantitation Range. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5. 1 Prepare a 2 μ g/mL stock solution of dsDNA in TE. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. Mit dem PicoGreen dsDNA. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. 2023-11. Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Triplicate 10 μL samples of λ DNA (Ο), E. Qubit® dsDNA HS Assay Kit. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. P11495. o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Figure 1. Bioz Stars score: 86/100, based on 1 PubMed citations. Unit Size. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されて. High tolerance for contaminants. Catalog Number. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. ru by: 1. Note: The 20X TE buffer (Component B) included in the PicoGreen dsDNA Quantitation Kit is certified to be nucleic acid-free and DNase-free. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. 5mL. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. 反应次数. Rapidly detect as little as 0. Katalognummer: P11496. 9. Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. One microgram of DNA was sheared using an M220. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. 5mL of TE x 5uL PicoReagent. 1 mL kit. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Rapidly detect as little as 0. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;DNA Quantification Using Quant-iT™ PicoGreen® The Quant-iT™ PicoGreen® assay was tested to overcome limitations of the UV absorbance assay at very low DNA concentrations. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Using 20x TE buffer make 1x solution. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. a) 16. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 995 mL TE for the . 5. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. For other nucleic acid methods (RiboGreen, OliGreen), quantified in thePicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. It must be completely liquified before use by allowing it to come to room temperature. no. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s.